Coding

Part:BBa_K1965043:Design

Designed by: Katja Leben   Group: iGEM16_Slovenia   (2016-10-17)


CRY:nPPV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 411
    Illegal BglII site found at 870
    Illegal BamHI site found at 1349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 295
    Illegal AgeI site found at 1024
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 647
    Illegal BsaI.rc site found at 56
    Illegal BsaI.rc site found at 1597
    Illegal BsaI.rc site found at 1777
    Illegal SapI.rc site found at 164
    Illegal SapI.rc site found at 1572


Design Notes

This sequence is inserted into pcDNA3 vector, appropriate for expression in mammal cell.


Source

CRY - Arabidopsis thaliana
nPPV - Plum pox virus

References